Coding

Part:BBa_K1152012:Design

Designed by: Ralf Beer, Konrad Herbst, Nikolaos Ignatiadis   Group: iGEM13_Heidelberg   (2013-09-20)

DelC 4'-Phosphopanthetheinyl-transferase

  • CDS of sfp derived from Delftia acidovorans SPH-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 288


Design Notes

The delC CDS is derived from Delftia acidovorans SPH-1. [Johnston, 2013] We cloned sfp in a pSB3K3-derived plasmid with a lac promoter (BBa_R0010) and the RBS BBa_B0029.


Source

We amplified delC from Delftia acidovorans SPH-1 obtained from DSMZ.

References

  • Johnston, C. W., Wyatt, M. a, Li, X., Ibrahim, A., Shuster, J., Southam, G., & Magarvey, N. a. (2013). Gold biomineralization by a metallophore from a gold-associated microbe. Nature chemical biology, 9(4), 241–243. doi:10.1038/nchembio.1179