Part:BBa_K1150003:Design

G9a

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 597
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part was PCR amplified from a plasmid containing the murine G9a set-domain (A. Jeltsch, Stuttgart). Primers with overhangs containing the RFC_25 prefix and suffix were used to insert iGEM restriction sites. To eliminate illegal PstI restriction sites within the coding region of G9a two point mutations were introduced via overhangs of overlapping primers and the G9a fragments were subsequently assembled using Gibson assembly.

Source

...