![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K1137011:Design
Designed by: Matthew Deyell Group: iGEM13_Paris_Bettencourt (2013-09-23)
sRNA anti lac
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 153
Illegal XhoI site found at 159 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Sequence of the sRNA must match exactly the target sequence for silencing. Therefore we needed to sequence the available lacZ to design our sRNA. If users lacZ has any point mutations, silent or otherwise, silencing may not occur.
Source
gBlock Synthesis with sRNA design from "Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli" Yoo et al 2013 Nature Protocols