Composite
Part:BBa_K1136005:Design
Designed by: Simin Jiang Group: iGEM13_Nevada (2013-09-16)
An L-Arabinose controlled endolysin from bacteriophage ΦKZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The endolysin gene was synthesized by GenScript to optimize codons and avoid illegal sites. The promotor and terminator were chosen for controllable expression, because the endolysin may be toxic in high copy number.
Source
The endolysin gene is from bacteriophage ΦKZ. An E. coli promoter and terminator were used.