Generator

Part:BBa_K1135001:Experience

Designed by: Kong Khai Jien   Group: iGEM13_UCL_PG   (2013-09-16)

Applications of BBa_K1135001

mKeima is generally a very good fluorescent protein with long, clean stoke shift and high brightness. Because of its red shifted emission spectrum it is an ideal choice for mammalian cell, tissue or animal related works.

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UNIQ71f077667379ffdc-partinfo-00000000-QINU UCL_PG expressed this biobrick in E.coli, 37C overnight in LB with Chloramphenicol. The culture is visibly fluorescence under blue light.

Further of that, we characterized the excitation and emission spectra of the expressed mKeima.

UNIQ71f077667379ffdc-partinfo-00000002-QINU