Generator

Part:BBa_K1135001:Design

Designed by: Kong Khai Jien   Group: iGEM13_UCL_PG   (2013-09-16)


mKeima down stream of strong constitutive promoter and strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression of mKeima is already visible during plating


Source

Gene synthesis and codon optimization for expression in human cells. Derived from published sequence (Henderson et al., JACS, 2009 - PMCID: PMC2754865).


References