Composite
Part:BBa_K1132046:Design
Designed by: iGEM Toulouse Group: iGEM13_INSA_Toulouse (2013-09-20)
R1-pLac-Bxb1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 591
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 348
Illegal BamHI site found at 865
Illegal XhoI site found at 952 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1504
Illegal NgoMIV site found at 1591
Illegal AgeI site found at 641 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 213
Illegal BsaI.rc site found at 1699
Design Notes
Assembly between BBa_K1132042 and BBa_K907000
Source
De novo synthesis and cloning assembly.
References
[http://www.ncbi.nlm.nih.gov.gate1.inist.fr/pubmed/22454498 Callura et al.]