Composite

Part:BBa_K1132046

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)

R1-pLac-Bxb1

Assembly between BBa_K1132042 and BBa_K907000.
Bxb1 integrase is under the control of a riboregulator device BBa_K1132042. It permits to precisely regulate the Bxb1 expression level through pTet and pLac inducible promoters. See BBa_K1132042 for further information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 591
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 348
    Illegal BamHI site found at 865
    Illegal XhoI site found at 952
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1504
    Illegal NgoMIV site found at 1591
    Illegal AgeI site found at 641
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 213
    Illegal BsaI.rc site found at 1699


[edit]
Categories
Parameters
None