Part:BBa_K1132045

Promoter T7-RFP

Coding sequence of RFP ( BBa_K081014) under control of T7 promoter (BBa_I712074). T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Then RFP will be produced only in the presence of T7 RNA Polymerase ( BBa_K1132000). The RFP coding sequence is flanked by a rbs and a terminator. Red color can be seen after 18 hours. Such a system permits strong and specific transcription of RFP.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 630
  Illegal AgeI site found at 741
  
• 1000
  COMPATIBLE WITH RFC[1000]

Results of BBa_K1132045 characterization

Following is the electrophoresis gel showing that we obtained a fragment of expected length (829 bp) by restriction with EcoRI/PstI:

Verifications of the validity of the constructions were performed with visual inspection of the colours of the colonies. The pT7-RFP plasmid was transformed into competent BL21-DE3. Plates were supplemented or not with IPTG. White colonies were obtained on the Petri dishes with no IPTG and red colonies on the Petri dishes containing IPTG.

The biobrick behave as expected.