Composite

Part:BBa_K1132037:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)


AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1141
    Illegal NheI site found at 1164
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 957
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 264
    Illegal AgeI site found at 376
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick is design to test the AND gate (BBa_K1132034) by measuring the level of RFP after recombination events. The biobrick Bba_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been inserted inside our gate between the FimE restrictions sites. The T7 polymerase gene is also present in the biobrick, under the control of a strong promoter, strong RBS. This part can therefore be used stand-alone as all elements to control the RFP output are present.


Source

Cloning assembly.

References

[http://www.ncbi.nlm.nih.gov/pubmed/23539178 Amplifying genetic logic gates. Bonnet et al.]
[http://www.ncbi.nlm.nih.gov/pubmed/23396014 Synthetic circuits integrating logic and memory in living cells. Siuti et al.]