Composite

Part:BBa_K1132032:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-19)

Test of the XOR gate (BBa_K1132002) with RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 122
    Illegal AgeI site found at 996
    Illegal AgeI site found at 1108
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 142
    Illegal BsaI.rc site found at 331


Design Notes

This BioBrick is design to test the XOR gate (BBa_K1132002) by measuring the level of RFP after recombination events. The biobrick Bba_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been assembled with our gate.

2032.png


Source

Cloning assembly.

References

[http://www.ncbi.nlm.nih.gov/pubmed/23539178 Amplifying genetic logic gates. Bonnet et al.]
[http://www.ncbi.nlm.nih.gov/pubmed/23396014 Synthetic circuits integrating logic and memory in living cells. Siuti et al.]