Composite
Part:BBa_K1132028:Design
Designed by: iGEM Toulouse Group: iGEM13_INSA_Toulouse (2013-09-20)
Bxb1 integrase coding sequence with RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 210
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 484
Illegal XhoI site found at 571 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1123
Illegal NgoMIV site found at 1210
Illegal AgeI site found at 260 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1318
Design Notes
Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). See BBa_K907000 for further infomation.
Source
BBa_B0034 (RBS),
BBa_K907000 (Bxb1 integrase)