Composite

Part:BBa_K1132027:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)

Bxb1 integrase coding sequence with constitutive weak promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 253
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 527
    Illegal XhoI site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1253
    Illegal AgeI site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1361


Design Notes

Bxb1 integrase allows DNA 180° switching between two recognition sites (attB and attP). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter. See BBa_K907000 for further infomation.


Source

BBa_B0034 (RBS),
BBa_K823013 (Pfaible),
BBa_K907000 (Bxb1 integrase)

References