Composite

Part:BBa_K1132025:Design

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)

FimE integrase coding sequence with constitutive weak promoter and terminator.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FimE integrase allows DNA 180° switching between two recognition sites (IRL : BBa_K137010 and IRR : BBa_K137008). As the protein is very active even at low level of expression, it is associated with a constitutive weak promoter.


Source

BBa_B1010 (term),
BBa_B0034 (RBS),
BBa_K823013 (Pfaible),
BBa_K137007 (FimE integrase)

References