Coding

Part:BBa_K1132012

Designed by: iGEM Toulouse   Group: iGEM13_INSA_Toulouse   (2013-09-20)

heme oxygenase (ho1), ferredoxin oxidoreductase (PcyA) and tetracycline repressor (TetR)

Two genes required for phycocyanobilin (PCB) biosynthesis and the coding region for the TetR protein.

ho1 produces biliverdin IXalpha, it represents the first of two steps in PCB biosynthesis. PcyA converts biliverdin IXalpha to PCB. PCB associates with EnvZ fusion protein, Cph8 (Part:BBa_I15010), to transduce a light signal into a genetic response.

TetR binds to the p(TetR) (Part:BBa_R0040) and inhibits its operation. It regulates the light sensor Cph8 (Part:BBa_K1132015). aTc (anhydrotetracycline) binds to TetR and allows Cph8 production.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1549
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1102
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None