Coding

Part:BBa_K1131007:Design

Designed by: Ramya Prathuri   Group: iGEM13_Berkeley   (2013-10-28)


mutant oleD 'ASP' glucosyltransferase; S. antibiotics (characterized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 688
    Illegal NgoMIV site found at 928
    Illegal NgoMIV site found at 1118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 387
    Illegal BsaI.rc site found at 653
    Illegal BsaI.rc site found at 1078


Design Notes

Source

Cloned from BBa_K1131006.

References

Gantt , Richard W. "Probing the Aglycon Promiscuity of an Engineered Glycosyltransferase." 47.46 (2008): 8889–8892. Print. .