Composite

Part:BBa_K113002:Design

Designed by: WU Jingjing, Wang Jinyu   Group: iGEM08_HKUSTers   (2008-10-23)

pLac->cI434->pT7->cI434 operator ->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 1604


Design Notes

We combined BBa_K113000 and BBa_K113001 by fusion PCR. Since the efficency is very low, we tried to use standard assembly to ligate them together.

Source

BBa_K113000 BBa_K113001

References