Coding
Part:BBa_K1129019:Design
Designed by: UBC iGEM 2013 Group: iGEM13_British_Columbia (2013-08-30)
Complete caffeine synthesis pathway under pTET constitutive promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1373
Illegal NheI site found at 1396
Illegal NheI site found at 2757
Illegal NheI site found at 2780 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2405
Illegal BglII site found at 3807
Illegal BglII site found at 3903 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We have yet to test for expression, and we may find that codon optimization is needed.
Source
The constitutive promoter we used was from the 2012 Distribution Kit, part BBa_J23118. The caffeine biosynthesis genes we assembled were from the 2013 Distribution Kit and were submitted by the 2012 TU Munich team: parts BBa_K801070, BBa_K801071, BBa_K801072.