Part:BBa_K1129014:Design

CaMXMT1 under pTET constitutive promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1039
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimization has not yet been performed, but we suspect it may be needed for successful expression in a prokaryotic system.

Source

The constitutive promoter comes from part BBa_J23118, found in Plate 1 of the 2012 Distribution Kit. The gene comes from TU Munich 2012 part BBa_K801071 in the 2013 Distribution Kit.