Tag
S tag

Part:BBa_K112704:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of <S-tag! basic part K112704

Wobble PCR of dv011/dv012		(60 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112704
----------------------------------------
dv011		Forward Biobricking of <S-Tag!
cgataGAATTCatgAGATCTaaagaaaccgctgctgctaaattcgaacgcc

dv012		Reverse Biobricking of <S-Tag!
cgttaGGATCCttagctgtccatgtgctggcgttcgaatttagcagc

References