Coding

Part:BBa_K1127006

Designed by: Jonas Kondratavicius   Group: iGEM13_York_UK   (2013-09-15)

mtrABC electron pump

Proteins A B and C of mtr complex put together. B and C complexes have rbs for each, mtrA however does not which allows selected transcription of this protein.

We optimized codon usage of the mtrA for efficient expression within E. coli. In addition, we removed legal sites from the mtrABC sequences (e.g. PstI in the mtrC). Please go to our iGEM wiki [http://2013.igem.org/Team:York_UK/Notebook.html?page=wetlab] for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3387


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Categories
Parameters
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