Tag

Part:BBa_K1125001:Design

Designed by: Tsung-Yu, Lu   Group: iGEM13_NTU_Taiwan   (2013-09-16)


Tandem Affinity Purification (TAP) tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 106
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 215
    Illegal BglII site found at 389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. receive pRS424-GAL1-EST1-TAP plasmid from Dr. Jin-Je Lin, National Taiwan University
2. clone the TAP fragment and remove the invalid EcoRI site by PCR.

For more design details, please check our team wiki page.

Source

artificial construct

References