Translational_Unit

Part:BBa_K1125000:Design

Designed by: Tsung-Yu, Lu   Group: iGEM13_NTU_Taiwan   (2013-09-16)


SrUCPa w/ kozack sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 46
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 57
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1. receive the pBS-SK+(SrUCP) plasmid from Dr. Kikokatsu Ito, Iwate university
2. cloned into pSB1C3 using 2 primers
f-primer: gtcaGAATTCgcggccgctTCTAGAgATCGAGGTTTCCCGG
r-primer: cgcaCTGCAGcggccgctACTAGTaTTATTAATTTGGCACCTCTTTG

3. an invalid XbaI site was removed by site-directed mutagenesis PCR using 4 primers.
f-primer: gtcaGAATTCgcggccgctTCTAGAgATCGAGGTTTCCCGG
mut-r-primer:
mut-f-primer:
r-primer: cgcaCTGCAGcggccgctACTAGTaTTATTAATTTGGCACCTCTTTG

For more design details, please check our team wiki page.

Source

cloned from genomic DNA of skunk cabbage, Symplocarpus renifolius.

References