Project

Part:BBa_K112408

Designed by: Christina Brown   Group: iGEM08_UC_Berkeley   (2008-10-23)

T4 phage derived lysis device

[T4 holin][T4 endolysin][dblTerm][T4 antiholin][rnpB T1] in T4 holin

T4 phage derived lysis device with holin, lysozyme and antiholin under a constitutive promoter

This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
    Illegal NheI site found at 1370
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 67
    Illegal NgoMIV site found at 1700
    Illegal AgeI site found at 953
    Illegal AgeI site found at 1023
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1602


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