Regulatory

Part:BBa_K112401:Design

Designed by: Christina Brown   Group: iGEM08_UC_Berkeley   (2008-10-21)

Promoter for recA gene - SOS and Ultrasound Sensitive


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pBca1256-K112401

Biobricking of promoter for recA protein 
 PCR recA-F and recA-R on MG1655 	  (317 bp, EcoR1/BamH1)
 Sub into pBca1256	                  (EcoR1/BamH1 910+2472bp, L)
 Product is pBca1256-K112401
 ----------------------------
cb0003-recA-F Forward Biobricking    cgataGAATTCatgAGATCTagagaagcctgtcggcaccg
cb0004-recA-R Reverse Biobricking    cgtagGGATCCcccagtgctgccgccaacgc


Source

Cloned from E. coli MG1655 genome.

References