Reporter
amilGFP (+

Part:BBa_K1124000:Design

Designed by: Yuta Otsuka   Group: iGEM13_UT-Tokyo   (2013-09-11)

amilGFP (+LVA), yellow reporter protein with degradation tag



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LVA degradation tag was added to increase protein degradation. .

13 amino acids (sequence: RPAANDENYALVA) and double stop codon were added. The first 2 aa (sequence: RP) is Stu1 restriction site, which may not be necessary to increase protein decay. LVA tag is the last 11 aa(sequence: AANDENYALVA) .1

Source

PCR (template: BBa_K592010, primers are shown on our wiki (UT-Tokyo 2013)(currently under construction). )

References

1 Andersen, J. B., Sternberg, C., Poulsen, L. K., Bjørn, S. P., Givskov, M., & Molin, S. (1998). New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Applied and environmental microbiology, 64(6), 2240-2246.