Composite

Part:BBa_K112368:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-28)


{Pbad}{rbs_ompT>} {< Cre>}{< S-Tag!}{< b1006>}


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 33
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 33
    Illegal NheI site found at 1184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 33
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 33
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 33
    Illegal AgeI site found at 1359
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1050


Design Notes

Digest (BgIII methylated) K112900 CA, (BamHI methylated) K112348 AK     (BamHI/BgIII/XHoI)
Product is pBjh1601CK-K112368


Source

BBa_K112900 and BBa_K112348


References