Intermediate

Part:BBa_K112344:Design

Designed by: Aron Lau   Group: iGEM08_UC_Berkeley   (2008-10-28)


{rbs_lamB>} {< ihfA>}{< phoA!}{< b1006>}


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 1052
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Digest (BgIII methylated) K112332 AC, (BamHI methylated) K112324 CK     (BamHI/BgIII/XHoI)
Product is pBjh1601AK-K112344


Source

BBa_K112332 and BBa_K112324


References