Translational_Unit

Part:BBa_K1122681:Design

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-30)


pSpac - LacZ - Fur


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 559


Design Notes

This part was generated with MABEL from K1122680 using standardised primers.

Presence of LacZ alpha peptide enables blue- white screening in E. coli when preparing for insertion into B. subtilis


Source

synthetic

References