Translational_Unit

Part:BBa_K1122679:Design

Designed by: Aleksandra Lewicka, Jan Lyczakowski   Group: iGEM13_Edinburgh   (2013-08-30)


pSpac - LacZ - fused pdc and adhB


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 899
    Illegal AgeI site found at 2105
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part was generated from EdiBrick - fused pdc and adhB (K1122674) via MABEL utilising standardised primers.

Presence of LacZ alpha peptide enables blue - white screening in E. coli when the part is inserted into an E. coli - B. subtilis shuttle vector.


Source

synthetic

References