Protein_Domain

Part:BBa_K112233:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


< phoA >


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 43
    Illegal NgoMIV site found at 490
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Construction of {<phoA>} Biobrick Part K112233
PCR mea036/mea037 on pBca9145-Bca1126		(1219 bp, EcoRI/BamHI)
Sub into pBjh1601AC-Bca1144			(EcoRI/BamHI, 3195,910 L)
Product is pBjh1601AC-K112233
----------------------------------------
mea036   Forward Biobricking of {<phoA>}	cgatagaattcatgAGATCTGGAGATTCTG
mea037   Reverse Biobricking of {<phoA>}	cgttaGGATCCCTTCAGGCCCAGCGCCGCTTTCATG


References