Part:BBa_K112201:Design

< T4 holin! in BBb

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

PCR bx3/bx2 on Bjh1301		(685 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112001
----------------------------------------
bx3		Forward biobricking of {<T4 Holin!}
ccagtGAATTCatgAGATCTgcagcacctagaatatcattttcgccc
bx2		Reverse biobricking of {<T4 Holin!}
cgttaGGATCCttatttagcccttcctaatattctggccgcc