Translational_Unit

Part:BBa_K112011:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-09-03)


rbs.T4 antiholin! in BBb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 220


Design Notes

N/A


Source

PCR bx12/bx8 on Bjh1303		(339 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256			(EcoRI/BamHI)
Product is pBca1256-K112011
----------------------------------------
bx12	Forward biobricking of {rbs.T4 Antiholin!}
ccagtGAATTCatgAGATCTgataggaggcctttatggccttaaaagcaacagc
bx8		Reverse biobricking of {rbs.T4 Antiholin!}
cgttaGGATCCtcattcagtctccaatttaatgttc

References