Coding

Part:BBa_K1114516:Design

Designed by: Jake Awtry   Group: iGEM13_BostonU   (2013-09-12)


Level 1 MoClo Device that expresses tetR under pBAD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pI134B2C40_AE This is a Level 1 MoClo device that expresses YFP under the repressible promoter lacI. It contains the Level 0 parts J23104_AB, BCD2_BC, C0040_CD, and B0015_DE within the DVL1_AE backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.


Source

Construct of MoClo Level 0 parts derived from iGEM Distribution Kits


References