DNA

Part:BBa_K1114035:Design

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-13)


pVioA in Moclo


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 34
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 34
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made primers to add AB Fusion sites. In pSB1C3 Backbone with SpeI sites added.

Source

HOLDEN LAB

References