Coding

Part:BBa_K1111010:Design

Designed by:   Group: iGEM13_EPF_Lausanne   (2013-09-19)


Streptavidin Alive


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 121
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 43
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 121
    Illegal AgeI site found at 85
    Illegal AgeI site found at 136
  • 1000
    COMPATIBLE WITH RFC[1000]


PCR

Primers used to amplify Streptavidin Alive coding sequence (384bp) from cloning plasmid:
5' ATGGCTGAAGCTGGTATCACC 3'
5' TTAGGAAGCAGCGGACGGTTTAAC 3'

Source

Can be expressed in Escherichia Coli.

References and Acknowledgements

Thanks to the Mark Howarth laboratory at Oxford university Dept. of Biochemistry [http://users.ox.ac.uk/~bioc0756/MyWebs/activesite/ReagentDistribution.htm] for providing us with the streptavidin cloning plasmid.