Composite
Part:BBa_K1111002:Design
Designed by: Luisa Spisak Group: iGEM13_EPF_Lausanne (2013-09-19)
Hya promoter and Superfolded GFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 184
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 184
Illegal NheI site found at 499 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 184
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 184
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 184
Illegal AgeI site found at 26 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 543
Design Notes
The Promoter was isolated directly from the Genomic DNA by PCR. In order to have the entire sequence, we took the sequence 500bp upstream until the start codon. It was then inserted into a Plasmid where it replaced the arabinose promoter.
Source
Escherichia Coli, K-12, Substrain MG1655
References
Journal of Bacteriology
Response of hya Expression to External pH in Escherichia coli
Paul W. King and Alan E. Przybyla
J.Bacteriol. 1990
Journal of Bacteriology
Mutational analysis and charecterization of the Escherichia coli hya operin, which encodes [NiFe] hydrogenase1
N K Menon, J Robbins, J C Wendt, K T Shanmuagam and A E Przybyla
J. Bacteriol 1991