Plasmid_Backbone

Part:BBa_K1110001:Design

Designed by: Lydia Studdard   Group: iGEM13_Georgia_State   (2013-09-17)


pGAPzalphaC+standardizing linker


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3126
    Illegal SpeI site found at 2
    Illegal PstI site found at 24
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3126
    Illegal SpeI site found at 2
    Illegal PstI site found at 24
    Illegal NotI site found at 13
    Illegal NotI site found at 3137
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3126
    Illegal BglII site found at 2362
    Illegal BamHI site found at 442
    Illegal XhoI site found at 3097
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3126
    Illegal SpeI site found at 2
    Illegal PstI site found at 24
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3126
    Illegal SpeI site found at 2
    Illegal PstI site found at 24
    Illegal NgoMIV site found at 1199
    Illegal NgoMIV site found at 1260
    Illegal AgeI site found at 161
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 425
    Illegal BsaI.rc site found at 624
    Illegal BsaI.rc site found at 1621
    Illegal SapI site found at 2605


Design Notes

NOne


Source

pGAPzalphC, Saccharomyces cerevisiae

References