Part:BBa_K1106004

GFP generator under Ars promoter

GFP generator under the control of an arsenite sensible promoter.

Usage and Biology

It can be used to quantify arsenic concentration in liquid samples by fluorescense measurement.

This parts was originally built to be used in [http://2013.igem.org/Team:Buenos_Aires iGEM BsAs 2013 project] to implement an arsenic sensor device based on synbio technology. [http://2013.igem.org/Team:Buenos_Aires Visit our wiki] to read details about the designing process and implementation details of our system. Also feel free to contact us for any question regarding this part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 255
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1191
  

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Contribution

Group: NCTU_Formosa 2017

Author: Yi-Ting Lin, Huei-Yu Yeh, Sheng-Ping Chu

New improved part Improvement

Summary

BBa_J33201 is a promoter containing the E. coli JM109 chromosomal arsenic detoxification operon (ars operon). Both 2013 Buenos Aires iGEM team and 2006 Edinburgh iGEM team used it to detect arsenic ions in the water. Buenos Aires team ligased it with GFP and Edinburgh team ligased it with LacZ α. The two teams also submitted biobricks, BBa_K1106004 and BBa_J33203. We wanted to know whether these two biobricks have more application, such as detecting the arsenic pollution in Chinese medicine. We also wanted to know whether they can detect other metal ions. Several experiments were done to evaluate the function of this promoter.