Regulatory
Part:BBa_K1104203:Design
Designed by: Ting-Yun Chiang Group: iGEM13_NYMU-Taipei (2013-09-16)
AhpCp1000
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers For PCR
| AhpCp1000_FP | gcttctagag tccatcagtttctcattgtcacttaacact |
| AhpCp1000_RP | gga ctgcagcggccgctactagta ctatacttcctccgtgttttcgatgaga |
| ahpC promoter(K362001)has a Pst1 site in the sequence. We designed this part AhpC(mutated), and succesfully mutated the Pst1 site (ctgcag->ctacag). | |
|---|---|
| ahpCp_mut_FP | cacctcagcctacaggcaggcactgaa |
| ahpCp_mut_RP | ttcagtgcctgcctgtaggctgaggtg |
PCR Time Protocol
| Temperature(°C) | Time(sec) | Number of Cycles |
|---|---|---|
| 94 | 120 | -- |
| 94 | 15 | 35 |
| 53 | 30 | |
| 72 | 68 | |
| 72 | 300 | -- |
Source
E. coli MG1655 genomic sequence