Part:BBa_K1104202:Experience

HemHp (Part:BBa_K1104202) is an OxyR-induced promoter, and was used as sensor to ROS(Reactive Oxygen Species).

HemH promoter +E0840 (Part:BBa_K1104242)

We designed the device: HemH promoter +E0840 (Part:BBa_K1104242) includes HemH promoter (Part:BBa_K1104242) and Part:BBa_E0840, induced HemH promoter by different concentrations of H₂O₂, and identified the strength.

In this device, after HemHp (Part:BBa_K1104202) is activated, GFP generator Part:BBa_E0840 takes as input a transcriptional signal (PoPS) and produce as output GFP (green fluorescent protein). We induced HemH promoter by different concentrations of H₂O₂, and measured the expression of GFP, and identified the strength.

Results

This is the relative promoter strength of BBa_K1104202 under different H₂0₂ concentrations tested using BBa_K11042042 (BBa_K1104202 +E0840) and found no significant trend where the higher the H₂0₂ concentration, the higher the promoter expression:

(Error bars represent the 95% confidence interval)

Strengths of the basal level of various promoters that respond to reactive oxygen species with respect to BBa_J23101:

Comparison between ahpC and its derived promoters.(Error bars represent the 95% confidence interval)

More details about ROS-induced promoters are in page: [http://2013.igem.org/Team:NYMU-Taipei/Project/Inhibition/Sensor Sensing Nosema ceranae].

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