Coding
Part:BBa_K1104200:Design
Designed by: Ting-Yun Chiang Group: iGEM13_NYMU-Taipei (2013-09-11)
OxyR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Primers For PCR
| OxyR_FP | gcttctag atgAATATTCGTGATCTTGAGTACCTGGTG |
| OxyR_RP | gga ctgcagcggccgctactagta ttaAACCGCCTGTTTTAAAACTTTATCGAAATGG |
PCR Time Protocol
| Temperature(°C) | Time(sec) | Number of Cycles |
|---|---|---|
| 94 | 120 | -- |
| 94 | 15 | 35 |
| 53 | 30 | |
| 72 | 60 | |
| 72 | 300 | -- |
Source
References
- Chiang, S. M., Schellhorn, H. E., & Catalases and Hydrogen Peroxide Metabolism. (September 15, 2012). Regulators of oxidative stress response genes in Escherichia coli and their functional conservation in bacteria. Archives of Biochemistry and Biophysics, 525, 2, 161-169.
- Vasil'eva, S. V., Iskandarova, K. A., & Makhova, E. V. (January 01, 1991). Oksidativnyĭ i SOS-otvety v Escherichia coli i ikh interferentsiia. Genetika, 27, 5, 809-19.
- D'Autréaux, B., & Toledano, M. B. (October 01, 2007). ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis. Nature Reviews Molecular Cell Biology, 8, 10, 813-824.