Measurement

Part:BBa_K1100152:Design

Designed by: YingQi Lou   Group: iGEM13_Fudan   (2013-09-16)

Csy4-16nt interaction estimation device


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 67
    Illegal SpeI site found at 885
    Illegal PstI site found at 1775
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 67
    Illegal SpeI site found at 885
    Illegal PstI site found at 1775
    Illegal NotI site found at 73
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 67
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 67
    Illegal SpeI site found at 885
    Illegal PstI site found at 1775
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 67
    Illegal XbaI site found at 82
    Illegal SpeI site found at 885
    Illegal PstI site found at 1775
    Illegal NgoMIV site found at 536
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 252


Design Notes

In this device, we test the interaction between csy4 and its 16-nt cleavage site in vivo to be a supplementary experiment for the Rachel E. Huarwitz et al, and Lei Qi et al's previous work.


Source

The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. sfGFP from Ding Yu's Lab at Fudan University, R0040, R0063 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, B0015 from Kit.


References

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.