Part:BBa_K1100152:Design
Csy4-16nt interaction estimation device
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 67
Illegal SpeI site found at 885
Illegal PstI site found at 1775 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 67
Illegal SpeI site found at 885
Illegal PstI site found at 1775
Illegal NotI site found at 73 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 67
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 67
Illegal SpeI site found at 885
Illegal PstI site found at 1775 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 67
Illegal XbaI site found at 82
Illegal SpeI site found at 885
Illegal PstI site found at 1775
Illegal NgoMIV site found at 536 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 252
Design Notes
In this device, we test the interaction between csy4 and its 16-nt cleavage site in vivo to be a supplementary experiment for the Rachel E. Huarwitz et al, and Lei Qi et al's previous work.
Source
The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. sfGFP from Ding Yu's Lab at Fudan University, R0040, R0063 from Kit, B0034 via PCR, Insulator: Csy4 loci via PCR, B0015 from Kit.
References
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.