Regulatory

Part:BBa_K1100002:Design

Designed by: Haotian Guo,Chenxi He   Group: iGEM13_Fudan   (2013-09-27)


J23100-ALeaderT-BCD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 169
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To validate the hypothesis that ALeaderT can be a functional terminator and then quantify the dose-termination curve.

Source

J23100 from Kit, AleaderT via de novo gene synthesis, BCD via PCR, mRFP from Kit.

References

Jia X, Zhang J, Sun W, et al. Riboswitch Control of Aminoglycoside Antibiotic Resistance[J]. Cell, 2013, 152(1): 68-81.

Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.

Haurwitz R E, Jinek M, Wiedenheft B, et al. Sequence-and structure-specific RNA processing by a CRISPR endonuclease[J]. Science, 2010, 329(5997): 1355-1358.

Mutalik V K, Guimaraes J C, Cambray G, et al. Precise and reliable gene expression via standard transcription and translation initiation elements[J]. Nature methods, 2013, 10(4): 354-360.