Protein_Domain

Part:BBa_K1095001:Design

Designed by: Quezia Keller Toe   Group: iGEM13_Leicester   (2013-09-23)

Protein Domain

TodX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Encodes the protein that facilitates the entry of toluene into the cells.

Design Notes

ORF was PCR amplified from Pseudomonas putida strain F1. Fusion PCR was used to add Biobrick prefix and suffix sequences. Fused PCR products were RCF10 cloned into pSB1C3. Insert sequence was confirmed by double stranded sequencing from flanking biobrick primers. Sequences submitted to Registry.

Source

Pseudomonas Putida F1 strain, NC_009512 from 3274514 (544 bp)

References

Parales, R.E., Parales, J.V., Pelletier, D.A., Ditty, J.L., 2008. Diversity of microbial toluene degradation pathways. Advances in Applied Microbiology. 64, 1-73, 2 p following 264.