Device

Part:BBa_K1092023

Designed by:   Group: iGEM13_Hong_Kong_CUHK   (2013-09-18)

T7-RBS-ssDsbA-PDZ Ligand-Voltage Switch-RFP C terminus

This is biobrick is the counterpart of the other biobrick T7-RBS-ssDsbA-PDZ Domain-Voltage sensor peptide-RFP N terminus (BBa_K1092024), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the RFP fragments (BBa_K1092105 & BBa_K1092106). In this Example, we demonstrate the possibility of using the voltage switch as a fast control of fluorescent signal. We made use of the biomolecular fluorescent complementation to achieve this purpose, and we worked on making the first reversible BiFC reaction in the world.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 405
    Illegal AgeI site found at 517
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None