Coding

Part:BBa_K1091005:Design

Designed by: Jian Huang   Group: iGEM13_UESTC   (2013-09-18)


pBAD-green fluorescent protein-SLOW


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 627
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Its nucleic sequence is designed to show more similarities with the Shine-Dalgarno sequence of E.coli via synonymous mutation using the Transpeeder software version 1.0.


Source

It has an identical amino acid sequence with the wild type green fluorescent protein of Aequorea victoria.

References