Device

Part:BBa_K1088014:Design

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-28)


E. coli dxs (lac promoter with lac inhibitor: IPTG inducible)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2158


Design Notes

All sequences derive from E. coli genome


Source

There is a scarsite between the RBS (BBa_B0030) and LacI aswell as a scarsite before and after the RBS of E. coli Dxs

References