Reporter

Part:BBa_K1088010:Design

Designed by: Andreas Kjær   Group: iGEM13_SDU-Denmark   (2013-08-20)


E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3937
    Illegal SapI.rc site found at 2127


Design Notes

The terminator aswell as the RBS and promoter was part of the reverse primer and forward primer for the LacI part, respectively. However we did insert a scar sequence between the RBS and startcodon to improve efficiency. There is also scar sites between the terminator (BBa_10002) and the rest of this composite part (BBa_K1088007). In the BBa_K1088007 part there is specific sites for scars aswell. Go to that parts page to investigate further.


Source

With the exception of the artificial terminator and the GFP part from BBa_K1088007 (GFP = BBa_E0040), everything derives from E. coli K-12 MG1655

References