Regulatory

Part:BBa_K1086000:Experience

Designed by: Ítalo Faria do Valle   Group: iGEM13_UFMG_Brazil   (2013-09-11)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1086000

This part is promoter of the TorCAD operon from Escherichia coli, and its expression only happens in response to Trimethylamine N-oxide (TMAO). The TMAO is a organic compound with the formula (CH3)3NO, and it is a metabolite produced by the liver in humans, but in bacteria TMAO is used to produce energy in the absence of oxygen. The TMAO binds to TorT protein, which activates the TorS protein. The TorS protein phosphorilates the TorR transcriptional factor, which binds to TorCAD promoter, activating the transcription.

User Reviews

UNIQb8bc9b3fbf91d7dc-partinfo-00000000-QINU UNIQb8bc9b3fbf91d7dc-partinfo-00000001-QINU